Corning® Matrigel®

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

• Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing


• Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process


• Tested and found negative for bacteria, fungi, and mycoplasma


• Protein concentrations are determined by Lowry method


• Endotoxin units are measured by Limulus Amoebocyte Lysate assay


• Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C


• Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor.

Source

Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application

• Cell Growth and Differentiation


• Metabolism/Toxicology Studies


• Invasion Assays


• in vitro and in vivo Angiogenesis Assays


• in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.